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mouse vegf quantikine colorimetric sandwich elisa kit  (R&D Systems)


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    R&D Systems mouse vegf quantikine colorimetric sandwich elisa kit
    Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and <t>VEGF</t> (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of <t>ELISA.</t> Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.
    Mouse Vegf Quantikine Colorimetric Sandwich Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 439 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse vegf quantikine colorimetric sandwich elisa kit/product/R&D Systems
    Average 96 stars, based on 439 article reviews
    mouse vegf quantikine colorimetric sandwich elisa kit - by Bioz Stars, 2026-03
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    1) Product Images from "The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells"

    Article Title: The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells

    Journal: Biology Open

    doi: 10.1242/bio.060081

    Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and VEGF (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of ELISA. Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.
    Figure Legend Snippet: Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and VEGF (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of ELISA. Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.

    Techniques Used: Knock-Out, Concentration Assay, Incubation, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Negative Control, Reverse Transcription, Western Blot



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    Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and <t>VEGF</t> (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of <t>ELISA.</t> Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.
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    Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and <t>VEGF</t> (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of <t>ELISA.</t> Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.
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    Image Search Results


    Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and VEGF (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of ELISA. Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.

    Journal: Biology Open

    Article Title: The autotaxin-LPA axis promotes membrane trafficking and secretion in yolk sac visceral endoderm cells

    doi: 10.1242/bio.060081

    Figure Lengend Snippet: Enpp2 knockout impairs secretion of angiogenic factors from the yolk sac. (A-B) Concentration of TGF-β1 (A) and VEGF (B) in the ECF of Enpp2 +/− and Enpp2 −/− embryos at E8.5 ( n =4 in [A], n =3 in [B], unpaired t -test). (C-D) Amount of TGF-β1 and VEGF secreted from the yolk sac. After the yolk sac was incubated for 16 h at 37°C in a culture medium, TGF-β1 and VEGF concentrations in the medium were measured by means of ELISA. Secretion of TGF-β1 and VEGF was reduced in the Enpp2 −/− yolk sacs ( n =3, unpaired t -test). (E) RT-PCR of Vegfa and Tgfb1 in the embryo and yolk sac of Enpp2 +/+ , Enpp2 +/− , and Enpp2 −/− at E8.5. RT- indicates a negative control in which a reverse-transcriptase was omitted from the reaction. (F) Western blot analysis of VEGF in the embryo and yolk sac of E8.5 mice. Abbreviation: n.s., not significant.

    Article Snippet: Amounts of VEGF-A and TGF-β1 in the ECF and yolk sac were determined by use of a mouse VEGF Quantikine Colorimetric Sandwich ELISA kit (R&D Systems) and a TGF-β1 E MAX ImmunoAssay System (Promega), respectively.

    Techniques: Knock-Out, Concentration Assay, Incubation, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Negative Control, Reverse Transcription, Western Blot